The Study of Extracellular Protein Fractions of Probiotic Candidate Bacteria on Cancerous Cell Line.
نویسندگان
چکیده
INTRODUCTION Probiotics are live microorganisms, habituated in the human intestine, which have a beneficial effect on our health. In spite of many reports about the anticancer effect of these bacteria in in-vivo and in-vitro, their mechanisms of action are not completely understood. The goal of this study was to compare the extracellular fractions of Lactobacillus casei and L. paracasei on the anti proliferation and apoptosis induction in K562 cell line. MATERIALS AND METHODS L. casei and L. paracasei were cultured in MRS broth medium. Then extracellular secretions were collected and after enrichment, analyzed by electrophoresis. Fractionation were determined by gel filtration chromatography using sephadex G100 column, and the anticancer properties were evaluated. RESULTS The results of SDS-PAGE showed various molecular weight of fractionated proteins of L. casei and L. paracasei. Bioactivity assessment illustrated that anti proliferative effects on K562 cells is dose and time dependent and the cytotoxic effects was parallel with protein concentration and the increase of time from 36 to 72 hours. CONCLUSION Regarding the cell cytotoxicity results, the fractionated extracellular proteins of L. casei and L. paracasei have significant effects in inhibition of cancer cell proliferation. However, more study is needed to better elucidate the mechanisms of extracted proteins, and its effect on other human cancer cell lines.
منابع مشابه
Cell-bound Exopolysaccharide Extract from Indigenous Probiotic Bacteria Induce Apoptosis in HT–29 cell-line
Background and Objective: The aim of this study was to compare the cytotoxic effects of local probiotic bacteria, including Lactobacillus paracasei, Lactobacillus brevis, while isolated from “Tarkhine” food and the induction of apoptosis in the HT–29 human colon adenocarcinoma cell line and normal fibroblasts. Methods: HT–29 and L–929 cell lines were trea...
متن کاملThe Study of Expression of Casp3/Bax Genes in HT29 Colon Cancer Cell Line with Lactobacillus rhamnosus Co-Culturing
Introduction: Colon cancer has spread to developed countries. Probiotic bacteria can play an important role in reducing cancer and treating colon cancer. The aim of this study was to determine the effect of adjacent bovine Lactobacillus rhamnose culture on the expression of casp3 and bax genes in cancer cell. Materials & Methods: In this experimental study after bacterial culture, supernatan...
متن کاملمقایسۀ توکسیسیتۀ فراوردۀ گیاهی SIM5 و فراکشنهای آن بر سلولهای طبیعی در حال استراحت، فعال و سلول سرطانی
Background and Objective: Common anticancer drugs have adverse effects due to targeting both normal and cancerous cells, so it seems valuable to search for further new anticancer compounds. Previously reported, herbal preparation SIM5 has toxic effect on cancerous cells, although its effect on normal proliferating cells is not clear. The present study was performed in order to further comparing...
متن کاملEvaluating the Effect of Protein A Signal Peptide on Extracellular Expression of Recombinant Hirudin in E.Coli
Abstract Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...
متن کاملSecreted Chemicals From Probiotic Bacteria Potentiate Th1 Pattern of Immune Cells and Apoptosis Induction in Breast Cancer and Gastric Adenocarcinoma Cell Lines
Cancer is one of the main causes of mortality. Therapeutic importance in some diseases, especially cancers has led to considering the anti-cancer effects of probiotics more than ever. Probiotics have stimulatory effects on the immunity and suppressor effects on cancer activity. Various studies report that probiotic bacteria have cytotoxicity effects on different types of solid tumors. In this r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Archives of Iranian medicine
دوره 19 11 شماره
صفحات -
تاریخ انتشار 2016